Rho抑制剂促进脊髓损伤小鼠脊髓轴突再生的研究
《创伤外科杂志》[ISSN:/CN:]
- 期数:
- 2011年03期
- 页码:
- 15
- 栏目:
- 基础研究
- 出版日期:
- 2011-07-30
文章信息/Info
- Title:
- Inhibitor of Rho can accelerate the functional recovery of hindlimb after spinal cord injury in rats
- Author(s):
- CHEN Jiajun; BAI Xiangjun; YI Chengla; et al.
- Department of Traumatic Surgery,Affiliated Tongji Hospital,Tongji Medical School of Huazhong University of Science and Technology, Wuhan430030，China
- 分类号:
- R651.2
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的观察损伤局部使用Rho抑制剂对脊髓损伤后小鼠轴突再生和神经功能恢复的作用。方法92只昆明小鼠分为4组做脊髓半切模型,对照组(20只)和3个实验组(每组24只)分别予以法舒地尔（Fasudil）、Y27632、胞外酶C3进行干预,术后第1、3、7、14、21、28天使用BBB评分法评估下肢运动功能。同时术后7、14、21、28天处死,取损伤节段下游脊髓冰冻切片免疫组织化学方法观察GAP43蛋白表达变化。结果前3周实验组下肢运动功能BBB评分均高于对照组,伤后实验组脊髓损伤节段下游生长相关蛋白43（GAP43）的表达高于对照组。结论Rho抑制剂可促进脊髓损伤的恢复,其机制尚需要进一步研究证实。
参考文献/References
［1］Dergham P,Ellezam B,Essagian C,et al.Rho signaling pathway targeted to promote spinal cord repair［J］.J Neurosci，2002,22(15):6570-6577.
［2］Yamashita T.Molecular mechanism and regulation of axon growth inhibition［J］.Brain Nerve，2007,59(12):1347-1353.
［3］Fournier AE,Takizawa BT,Strittmatter SM.Rho kinase inhibition enhances axonal regeneration in the injured CNS［J］.J Neurosci，2003,23(4):1416-1423.
［4］肖卫东,易成腊，陈安民，等.神经生长导向因子Slit2在成年大鼠急性脊髓损伤后的表达［J］.中华实验外科杂志，2006,23(8):987-989.
［5］Schwab JM,Conrad S,Elbert T,et al.Lesional RhoA+ cell numbers are suppressed by antiinflammatory,cyclooxygenaseinhibiting treatment following subacute spinal cord injury［J］.Glia，2004,47(4):377-386.
［6］Erschbamer MK,Hofstetter CP,RhoA OL,et al.RhoA,RhoB,RhoC,Rac1,Cdc42,and Tc10 mRNA levels in spinal cord,sensory ganglia,and corticospinal tract neurons and longlasting specific changes following spinal cord injury［J］.J Comp Neurol，2005,484(2):224-233.
［7］Madura T,Yamashita T,Kubo T,et al.Activation of Rho in the injured axons following spinal cord injury［J］.EMBO Rep，2004,5(4):412-417.
［8］Kubo T,Endo M,Hata K,et al.Myosin IIA is required for neurite outgrowth inhibition produced by repulsive guidance molecule［J］.J Neurochem，2008,105(1):113-126.
［9］Bito H,Furuyashiki T,Ishihara H,et al.A critical role for a Rhoassociated kinase,p160ROCK,in determining axon outgrowth in mammalian CNS neurons［J］.Neuron,2000,26(2):431-441.
［10］Wu CT,Wong CS,Yeh CC,et al.Treatment of cerebral vasospasm after subarachnoid hemorrhage：a review［J］.Acta Anaesthesiol Tai wan,2004,42(4):215 -222.
［11］Schaafsma D,Gosens R,BosI S,et al.Role of contractile prostaglandins and Rhokinase in growth factor-induced airway smooth muscle contraction［J］.Respir Res,2005,6(1):85.
［12］王晓英,张均田.突触可塑性与相关蛋白研究进展［J］.中国药理学通报,2001,17(4):369-372.
［13］Chan CC,Khodarahmi K,Liu J,et al.Dosedependent beneficial and detrimental effects of ROCK inhibitor Y27632 on axonal sprouting and functional recovery after rat spinal cord injury［J］.Exp Neurology,2005,196(2):352-364.
［14］Nishio Y,Koda M,Kitajo K,et al.Delayed treatment with Rhokinase inhibitor does not enhance axona regeneration or functional recovery after spinal cord injury in rats［J］.Exp Neurology,2006,200(2):392-397.
备注/Memo
- 备注/Memo:
- -
