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GFAP重组蛋白表达纯化及其主动免疫对脊髓损伤的神经保护作用

《创伤外科杂志》[ISSN:/CN:]

期数:
2011年03期
页码:
14
栏目:
基础研究
出版日期:
2011-07-30

文章信息/Info

Title:
Neuroprotective effect of active immunity with purified recombinant protein GFAP on spinal cord injury
作者:
曹鹏; 李志清; 高旭; 陈君; 张海峰; 梁国标
110015辽宁 沈阳，沈阳军区总医院神经外科通讯作者：梁国标，电话：(024)28856277，Email：liangguobiao6708@163.com
Author(s):
CAO Peng; LI Zhiqing; GAO Xu; et al.
Department of Neurosurgery，Shenyang General Hospital of PLA，Shenyang110015，China
关键词:
脊髓损伤; 胶质原纤维酸性蛋白; 质粒构建; 免疫; 神经保护
分类号:
R651.2
DOI:
-
文献标识码:
A
摘要:
目的构建表达胶质原纤维酸性蛋白（glial fibillary acidic protein，GFAP）的质粒，诱导表达GFAP蛋白，纯化鉴定后主动免疫大鼠，并通过SCI大鼠行为学研究GFAP蛋白主动免疫在脊髓损伤（spinal cord injury,SCI）中的神经保护作用，为开展下一步研究工作做准备。方法从人脑胶质瘤组织提取mRNA,采用RTPCR的方法克隆GFAP序列并表达后，将GFAP基因片段与原核表达质粒pGEX4T2连接，转化宿主菌BL21构建重组质粒，诱导表达后纯化，主动免疫大鼠，通过建立SCI大鼠模型，观察行为学评分，验证蛋白免疫效果。结果从脑胶质瘤组织克隆出1300bp的GFAP目的片段，成功构建GFAP表达质粒，制备并纯化GFAP重组蛋白后成功免疫大鼠，并通过SCI大鼠行为学评分验证其神经保护作用。结论成功构建重组质粒pGEMT/GFAP，表达纯化GFAP重组蛋白，并验证了GFAP蛋白主动免疫对脊髓损伤的神经保护作用。

参考文献/References

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备注/Memo

备注/Memo:
-
更新日期/Last Update: 2011-07-07