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载去铁敏的3D打印支架修复兔桡骨临界尺寸骨缺损实验研究

《创伤外科杂志》[ISSN:/CN:]

期数:
2020年12期
页码:
919-922+935
栏目:
论著·基础研究
出版日期:
2020-12-20

文章信息/Info

Title:
Experimental study on a 3D printed scaffold loaded with DFO in repairing critical size bone defect
文章编号:
1009-4237(2020)12-0919-05
作者:
崔 翔; 李 明; 刘鐘阳; 孙国飞; 刘 真; 刘建恒
100853 北京,中国人民解放军总医院第一医学中心骨科,国家骨科与运动康复临床医学研究中心(崔翔,李明,刘鐘阳,孙国飞,刘建恒); 100161 北京,中国人民解放军京南医疗区(刘真)
Author(s):
CUI Xiang1; LI Ming1; LIU Zhong-yang1; SUN Guo-fei1; LIU Zhen2; LIU Jian-heng1
1.Department of Orthopedics,The First Medical Center,Chinese PLA General Hospital,National Clinical Research Center for Orthopedics,Sports Medicine & Rehabilitation,Beijing 100853,China; 2.Beijing Southern Medical District,Chinese PLA,Beijing 100161,China
关键词:
桡骨骨缺损;  3D打印支架;  去铁敏;  修复;  兔
分类号:
R 683.41
DOI:
10.3969/j.issn.1009-4237.2020.12.009
文献标识码:
A
摘要:
目的 探讨载去铁敏(DFO)的3D打印支架修复兔桡骨临界尺寸骨缺损的效果。方法 采用3D打印技术制备载DFO的骨修复支架,扫描电镜观测支架表面形貌。体外实验中,细胞增殖实验验证其细胞相容性,qPCR方法验证支架促MC3T3-E1细胞成骨分化性能; 体内实验中,选取成年新西兰大白兔24只,雄性15只,雌性9只,兔龄15～18个月,体重2.5～3.0kg。随机分为空白组(单纯骨缺损)、对照组(空白3D打印支架)、载药组(载DFO的3D打印支架),每组8只。构建兔桡骨临界尺寸骨缺损模型后,分别植入相应支架,于术后即刻和12周时X线观察缺损处愈合情况,并在12周时处死实验兔,对肝肾进行HE染色观察组织相容性。结果 扫描电镜显示对照组、载药组3D打印支架形态均匀。体外实验中,细胞增殖实验显示支架细胞相容性好,载DFO的3D打印支架可促MC3T3-E1细胞成骨分化。体内实验证实,在12周时载药组骨缺损修复效果良好,且组织相容性好。结论 载DFO的3D打印支架具有良好的促临界尺寸骨缺损修复效能,具有潜在的应用前景。

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备注/Memo

备注/Memo:
【基金项目】 国家自然科学基金青年项目(81702153; 81702121); 军队医学科技青年培育计划孵化项目(19QNP052); 军队后勤科研项目重大项目分课题(AWS17J004) 【通信作者】 刘建恒,E-mail:jianhengliu@126.com
更新日期/Last Update: 2020-12-20