MK-801对改善实验性创伤性脑损伤后脑细胞线粒体呼吸作用的研究
《创伤外科杂志》[ISSN:/CN:]
- 期数:
- 2013年15卷05期
- 页码:
- 436-439
- 栏目:
- 基础研究
- 出版日期:
- 2013-11-24
文章信息/Info
- Title:
- Effects of MK-801 on the brain mitochondrial respiratory function in experimental traumatic brain injury
- Author(s):
- DAI Wenguang; QIN Xiaoyong; LI Lianghui; SONG Xueliang; FENG Yue; LI Zhen
- Department of Neurosurgery,253 Hospital of PAL,Huhhot 010051,China
- 分类号:
- R 651.15
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 【摘要】目的 观察N-甲基-D-天门冬氨酸（N-mechy1 D-aspartate，NMDA）受体拮抗剂（MK801液）对创伤后大鼠脑线粒体呼吸功能的影响，并探讨可能的作用机制，为临床应用MK801治疗创伤性颅脑损伤提供依据。方法 将Wistar大鼠80只随机分为4组：对照组20只，创伤未治组（非治疗组）20只，治疗24h组（治疗Ⅰ组）20只，治疗72h组（治疗Ⅱ组）20只。采用BIMⅢ型小型多功能生物撞击机造成大鼠中型颅脑损伤，用氧电极法分析线粒体呼吸功能（呼吸Ⅲ、Ⅳ态和呼吸控制率），并行透视电镜观察线粒体超微结构。结果 （1）与对照组比较，非治疗组伤后72h呼吸Ⅲ态和呼吸控制率明显下降；治疗Ⅰ组呼吸功能有所恢复；治疗Ⅱ组呼吸Ⅲ态和呼吸控制率较治疗疗Ⅰ组有显著升高（P<0.05），呼吸Ⅳ态稍有延长，但相差不显著。（2）与对照组比较，电镜结果示伤后非治疗组与治疗Ⅰ组、Ⅱ组线粒体结构均有不同程度的损害，但治疗Ⅰ组、Ⅱ组线粒体损害程度减轻。结论 创伤性颅脑损伤后脑线粒体呼吸功能明显下降。应用MK801治疗颅脑损伤大鼠可明显改善脑线粒体呼吸功能，电镜观察也发现线粒体超微结构损害程度减轻。治疗时间越长，呼吸功能改善越明显。
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备注/Memo
- 备注/Memo:
- -
