瞬时高加速度致舱内大鼠早期空间记忆障碍及其意义
《创伤外科杂志》[ISSN:/CN:]
- 期数:
- 2010年06期
- 页码:
- 487-490
- 栏目:
- 论著
- 出版日期:
- 2010-12-30
文章信息/Info
- Title:
- Transient, high acceleration induces early deficits in spatial memory of rats in airtight cabin
- 作者:
- 张子焕；赖西南；许民辉等
- 400042重庆，第三军医大学大坪医院野战外科研究所神经外科，创伤、烧伤与复合伤国家重点实验室通信作者：赖西南,许民辉,电话：（023）68757463，Email: laixinan@sohu.com
- Author(s):
- ZHANG Zihuan; LAI Xinan; XU Minhui; WANG Lili; XU Mingwei; LI Xiaoxia
- State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research,Department of Neurosurgery,Daping Hospital, Third Military Medical University, Chongqing400042,China
- 分类号:
- R322.81；R651.1
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的观察爆炸致密闭舱室内大鼠瞬时高加速度负载后早期空间记忆变化，探讨其相应的临床意义。方法制作爆炸致密闭舱室内大鼠（坐姿）瞬时高加速度负载时脑损伤模型；24只SD雄性大鼠随机分为正常对照组、800mgTNT爆炸当量致伤组（致伤组）、800mgTNT爆炸当量致伤+座位悬空组（减震组），每组8只；利用wavebook516A数据采集系统记录舱室内座位加速度大小，作为大鼠加速度负载评价指标；伤前7天为Morris水迷宫定位航行训练期，伤后1小时进行空间探索实验，记录大鼠穿越平台次数（The number of times of crossing the platform）与跨越目标象限时间占整个游泳时间的百分率（The percentage of the time spent on the target quadrant to the total swimming time）；空间探索实验结束后大鼠被处死,取脑固定，尼氏染色，观察海马CA1区锥体细胞损伤情况。结果致伤组座位加速度峰值为（13109±1167）g，作用时间为（11.08±1.43）ms，减震组座位加速度峰值仅为（4.0±1.2）g，作用时间仅为（0.34±0.08）ms；穿越平台次数：对照组（4.75±1.75）次，减震组（3.88±1.13）次，两者无明显差异（P>0.05），而与致伤组（1.13±0.64）次存在显著差异（P<0.01）；跨越目标象限时间占总游泳时间的百分率：对照组（53.93±13.72）%，减震组（49.60±10.75）%，两者无明显差异（P>0.05），而与致伤组（22.20±10.82）%存在显著差异（P<0.01）；尼氏染色示致伤组海马CA1区存在锥体细胞急性损伤或坏死性改变，而减震组、对照组则无此改变。结论爆炸致密闭舱室内大鼠瞬时高加速度负载后早期可出现空间记忆障碍，这一结果有助于提高对装甲车辆触雷爆炸、底板未击穿时乘员仍可能遭受轻度创伤性颅脑损伤（mildTBI）的认识，对临床医生来说具有重要意义。
参考文献/References
［1］Owen S.Armoured fighting vehicle casualties： 1977［J］.J R Army Med Corps, 2007,153(3):210-215.
［2］Dougherty PJ.Armored vehicle crew casualties［J］.Mil Med,1990,155(9):417-420.
［3］Tabiei A,Nilakantan G.Axial crushing of tubes as an energy dissipating mechanism for the reduction of acceleration induced injuries from mine blasts underneath infantry vehicles［J］.Int J Impact Engineering,2009,36(5):729-736.
［4］Boyd SD.Acceleration of a plate subject to explosive blast loading: trial results［R/OL］.(2009-09-15)
［2000-03］http://hdl.handle.net/1947/3552.
［5］宋博,胡时胜.进入密闭容器冲击波超压的实验研究［J］.弹道学报,2001,13(3):68-72.
［6］Mckay BJ,Bir CA.Lower extremity injury criteria for evaluating military vehicle occupant injury in underbelly blast events［J］.Stapp Car Crash J,2009,53:229-249.
［7］Ragel BT,Allred C,Brevard S,et al.Fractures of the thoracolumbar spine sustained by soldiers in vehicles attacked by improvised explosive devices［J］.Spine,2009,34(22):2400-2405.
［8］Okie S.Traumatic brain injury in the war zone［J］.N Engl J Med,2005,352(20):2043-2047.
［9］Gawande A.Casualties of warmilitary care for the wounded from Iraq and Afghanistan［J］.N Engl J Med,2004,351(24):2471-2475.
［10］Murray CK,Reynolds JC,Schroeder JM,et al.Spectrum of care provided at an echelon II medical unit during operation iraqi freedom［J］.Mil Med,2005,170 (6):516-520.
［11］Coupland RM,Meddings DR.Mortality associated with use of weapons in armed conflicts,wartime atrocities,and civilian mass shootings: literature review［J］.BMJ,1999,319(7207):407-410.
［12］Gondusky JS,Peiter MP.Protecting military convoys in Iraq: an examination of battle injuries sustained by a mechanized battalion during Operation Iraqi Freedom II［J］.Mil Med,2005,170(6):546-549.
［13］Warden DL,French L.War neurotrauma: the defense and veterans brain injury center (DVBIC) experience at walter reed army medical center (WRAMC)［J］.J Neurotrauma,2005,22(10):1178.
［14］Okie S.Reconstructing lives： a tale of two soldiers［J］.N Engl J Med,2006,355(25):2609-2615.
［15］Bhattacharjee Y.Shell shock revisited: solving the puzzle of blast trauma［J］.Science,2008,319(5862):406-408.
备注/Memo
- 备注/Memo:
- 全军“十一五”医学专项指令性课题(06Z034)；国家科技支撑计划专项(2009BAI87B00)
