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促肾上腺皮质激素释放激素基因敲除对烧伤后早期小鼠巨噬细胞趋化和组织细菌移位的影响

《创伤外科杂志》[ISSN:/CN:]

期数:
2010年04期
页码:
319
栏目:
论著
出版日期:
2010-08-31

文章信息/Info

Title:
Effects of burn injury on chemotaxis in peritoneal macrophages and bacteria translocation in tissues in corticotropin releasing hormone deficient mice
作者:
杨策; 王海燕; 黄苏娜; 严军; 钟河江; 刘庆; 李磊; 蒋建新
400042重庆，第三军医大学大坪医院野战外科研究所，全军交通医学研究所，创伤、烧伤与复合伤国家重点实验室
Author(s):
YANG Ce; WANG Haiyan; HUANG Suna; YAN Jun; ZHONG Hejiang; LIU Qing; LI Lei; JIANG Jianxin
(State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital,Third Military Medical University, Chongqing400042,China)
关键词:
创伤; 神经内分泌反应; 免疫; 激素; 细菌移位
分类号:
R 64；R 392
DOI:
-
文献标识码:
A
摘要:
目的观察促肾上腺皮质素释放激素(CRH)基因敲除的烧伤小鼠腹腔巨噬细胞趋化功能变化及其与组织细菌移位的关系。方法选用3种CRH基因型:野生型（+/+）,杂合子（+/-）,纯合子（-/-）小鼠，以凝固汽油复制20%的Ⅲ度烧伤模型，伤后6小时和24小时分别以腹腔灌洗法提取腹腔巨噬细胞，观察其趋化功能变化；并以血琼脂平板培养法，观察肝、肺和肠系膜淋巴结细菌移位情况。结果CRH-/-小鼠烧伤后，腹腔巨噬细胞趋化功能较CRH+/+烧伤小鼠显著减弱，伤后6小时趋化指数(14.05±5.54)低于伤后野生型(27.1±8.61)(P<0.05)，CRH-/-小鼠肝、肺和肠系膜淋巴结细菌菌落数显著高于CRH+/+和CRH+/-烧伤小鼠，以肠系膜淋巴结最为显著(P<0.01)；伤后24小时CRH-/-小鼠肠系膜淋巴结菌落数(974±356.75)和细菌移位率(100%)分别显著高于野生组小鼠肠道菌落数(574±191.4)和细菌移位率(40%)(P<0.01)，伴肠道炎性损伤加重。结论创伤应激早期，CRH基因敲除显著削弱机体天然免疫功能。

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备注/Memo

备注/Memo:
-
更新日期/Last Update: 1900-01-01