亚硝基谷胱甘肽对舱室内大鼠颅脑爆炸伤后继发性脑损伤的作用
《创伤外科杂志》[ISSN:/CN:]
- 期数:
- 2010年06期
- 页码:
- 491-495
- 栏目:
- 论著
- 出版日期:
- 2010-12-30
文章信息/Info
- Title:
- Effects of SNitrosoglutathione on secondary brain injury after explosive brain injury in enclosed space in rats
- 作者:
- 许明伟；许民辉；赖西南等
- 400042重庆，第三军医大学大坪医院野战外科研究所神经外科，国家创伤、烧伤与复合伤重点实验室通讯作者:许民辉,赖西南,电话：（023）68757463，Email: laixinan@sohu.com
- Author(s):
- XU Mingwei; XU Minhui; LAI Xinan; WANG Lili; ZHANG Zihuan; CUI Hong
- State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Surgery Research,Department of Neurosurgery,Daping Hospital,Third Military Medical University,Chongqing400042,China
- 分类号:
- R651.1
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的观察S亚硝基谷胱甘肽（GSNO）在舱室内大鼠颅脑爆炸伤后继发性脑损伤中的作用，探讨其在舱室颅脑爆炸伤防治中的临床应用价值。方法88只SD大鼠完全随机分为正常对照组（n=8）、单纯致伤组（n=40）和致伤+GSNO治疗组（n=40）；采用二硝基重氮酚（DDNP）纸质点爆源在模拟装甲舱室爆炸，建立颅脑爆炸伤模型。伤后1、6、12、24、48小时取标本。测定脑组织中肿瘤坏子因子α（TNFα）、白介素1β（IL1β）浓度；丙二醛（MDA）浓度及超氧化物岐化酶（SOD）活性；观察脑组织病理学变化。结果致伤组伤后1小时脑组织TNFα、IL1β、MDA浓度均明显升高（P<0.01），伤后12小时升高达峰值（P<0.01），伤后48小时脑组织TNFα、IL1β浓度仍显著高于正常对照组（P<0.01）。致伤组伤后1小时脑组织SOD活性即显著降低（P<0.01），致伤后12小时降低至最低值（P<0.01），伤后48小时仍低于正常值。致伤组脑血管内皮细胞肿胀、脑水肿、神经元变性、坏死明显；给予GSNO治疗后各时间点脑组织TNFα、IL1β、MDA浓度均较致伤组明显降低，SOD活性明显增高（P<0.01或P<0.05）。治疗组脑水肿、神经元变性、坏死等表现均较单纯致伤组轻。结论舱室颅脑爆炸伤后可导致大鼠严重的继发性脑损伤；而早期给予GSNO可明显减轻脑组织炎性损伤和氧化损伤，减轻脑水肿及神经元变性、坏死，从而减轻继发性脑损伤。
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备注/Memo
- 备注/Memo:
- 全军“十一五”医学专项指令性课题(06Z034)；国家科技支撑计划专项 (2009BAI87B00)
